Quantitative Proteomic Analysis of Chromatin-Associated Factors

J Am Soc Mass Spectrom. 2003 Jul;14(7):696-703. doi: 10.1016/S1044-0305(03)00204-6.

Abstract

A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • B-Lymphocytes / chemistry
  • B-Lymphocytes / cytology
  • Cell Line
  • Chromatin / chemistry*
  • Chromosomal Proteins, Non-Histone / analysis
  • Chromosomal Proteins, Non-Histone / genetics
  • Genes, myc / genetics
  • Humans
  • Nuclear Proteins / analysis*
  • Nuclear Proteins / genetics
  • Proteome / analysis*
  • Proteome / genetics
  • Up-Regulation

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Nuclear Proteins
  • Proteome