Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides

Michael Jahn; Dominik Stoll; R Antony J Warren; Lóránd Szabó; Pritpal Singh; Harry J Gilbert; Valérie M A Ducros; Gideon J Davies; Stephen G Withers

doi:10.1039/b302380j

Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides

Chem Commun (Camb). 2003 Jun 21:(12):1327-9. doi: 10.1039/b302380j.

Authors

Michael Jahn¹, Dominik Stoll, R Antony J Warren, Lóránd Szabó, Pritpal Singh, Harry J Gilbert, Valérie M A Ducros, Gideon J Davies, Stephen G Withers

Affiliation

¹ Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.

PMID: 12841226
DOI: 10.1039/b302380j

Abstract

Mutant endo-mannanases, in which the catalytic nucleophile has been replaced, function as glycosynthases in the synthesis of manno-oligosaccharides of defined lengths.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Catalysis
Cellulases / chemistry
Glycopeptides / biosynthesis*
Glycopeptides / chemistry
Glycoside Hydrolases / chemistry*
Glycosylation
Hydrogen-Ion Concentration
Mannans / chemistry
Mannosidases / chemistry*
Models, Molecular
Molecular Sequence Data
Multienzyme Complexes / chemistry*
Mutagenesis, Site-Directed
Oligosaccharides / biosynthesis*
Oligosaccharides / chemistry
Time Factors
Transferases / chemistry*

Substances

Glycopeptides
Mannans
Multienzyme Complexes
Oligosaccharides
transglycosidase enzyme system
mannobiose
Transferases
Cellulases
Glycoside Hydrolases
Mannosidases
endo-beta-mannosidase