Suppressive effects of E3330, a novel quinone derivative, on tumor necrosis factor-alpha generation from monocytes and macrophages

Agents Actions. 1992 Nov;37(3-4):297-304. doi: 10.1007/BF02028123.

Abstract

E3330 [(2E)-3-[5-(2,3-dimethoxy-6-methyl-1,4-benzoquinoyl)]-2-nonyl-2- propenoic acid], a novel synthesized hepatoprotective compound, has suppressive effects on tumor necrosis factor-alpha (TNF-alpha) generation from monocytes/macrophages in vitro. E3330 (1-100 microM) reduced lipopolysaccharide (LPS, 10 mg/ml or 1 microgram/ml)-induced TNF-alpha generation from rat resident and Propionibacterium acnes (P. acnes)-elicited peritoneal macrophages, rat and human monocytes, rat Kupffer cells, and splenic mononuclear cells in a concentration-dependent manner. E3330 also (1-100 microM) suppressed TNF-alpha generation stimulated with egg-albumin immune complex in rat P. acnes-elicited peritoneal macrophages. Northern blot analysis showed that LPS-induced expression of TNF-alpha messenger RNA (mRNA) in human blood monocytes was suppressed by E3330. These findings indicate that E3330 has a suppressive effect on TNF-alpha generation from monocytes/macrophages, regardless of origin or species, and this effect is based in part on the suppression of TNF-alpha mRNA expression.

MeSH terms

  • Animals
  • Base Sequence
  • Benzoquinones / pharmacology*
  • Blotting, Northern
  • Cells, Cultured
  • Depression, Chemical
  • Humans
  • Kupffer Cells / drug effects
  • Kupffer Cells / metabolism
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Propionates / pharmacology*
  • Propionibacterium acnes / immunology
  • RNA / isolation & purification
  • RNA / metabolism
  • Rats
  • Rats, Inbred F344
  • Spleen / cytology
  • Spleen / drug effects
  • Tumor Necrosis Factor-alpha / biosynthesis*

Substances

  • Benzoquinones
  • Lipopolysaccharides
  • Propionates
  • Tumor Necrosis Factor-alpha
  • E 3330
  • RNA