Abstract
A common structure of substrates of lignostilbenedioxygenases was investigated using synthesized stilbenes. Cell-free extracts of Sphingomonas paucimobilis TMY1009 degraded only trans-4-hydroxystilbene and trans-4-hydroxy-3-methoxystilbene. Other stilbenes that had no 4-hydroxyl group and had a cis structure were not substrates for lignostilbenedioxygenases. These results indicate that a 4-hydroxyl group and trans-structure is necessary for the common structure for substrates of lignostilbenedioxygenases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Extracts
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Chromatography, High Pressure Liquid
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Dioxygenases*
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Isoenzymes / chemistry*
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Isoenzymes / metabolism
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Ligands
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Oxygenases / chemistry*
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Oxygenases / metabolism
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Sphingomonas / enzymology*
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Stereoisomerism
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Stilbenes / chemistry
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Stilbenes / metabolism
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Structure-Activity Relationship
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Substrate Specificity
Substances
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Cell Extracts
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Isoenzymes
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Ligands
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Stilbenes
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4-hydroxystilbene
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Oxygenases
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Dioxygenases
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lignostilbene-alpha,beta-dioxygenase