Molecular cloning of quinohemoprotein alcohol dehydrogenase, ADH IIB, from Pseudomonas putida HK5

Biosci Biotechnol Biochem. 2003 Jun;67(6):1397-400. doi: 10.1271/bbb.67.1397.

Abstract

Molecular cloning of the gene of quinohemoprotein alcohol dehydrogenase (ADH IIB) from Pseudomonas putida HK5 was done. The gene (qbdA) was 690 amino acids in length, containing a 22-amino acid signal sequence. Another gene (qbdB) upstream of qbdA, probably in the same transcriptional unit, was found. Further upstream, a gene divergently transcribed against qbdBA had identity with NAD-dependent aldehyde dehydrogenase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Chromosome Mapping
  • Cloning, Molecular / methods*
  • Gene Components
  • Molecular Sequence Data
  • Multigene Family
  • Pseudomonas putida / enzymology*

Substances

  • Bacterial Proteins
  • Alcohol Oxidoreductases
  • alcohol dehydrogenase (acceptor)