The determination of immune reactive proteins in Cysticercus tenuicollis cyst fluids by SDS-PAGE and western blotting in sheep

Parasite. 2003 Jun;10(2):141-5. doi: 10.1051/parasite/2003102141.

Abstract

Cysticercus tenuicollis, the larva of Taenia hydatigena, is commonly seen in sheep and goats slaughtered in Turkey. In this study, the protein bands were revealed in C. tenuicollis cyst fluid antigen by using SDS-PAGE and Western blotting, and immune reactive bands were determined. Ten positive and 10 negative sera for C. tenuicollis from sheep and one non-infected sheep serum were tested in this experiment. According to the results, there was only one protein band determined to be immune reactive, which was 36 kDa.

MeSH terms

  • Animals
  • Antigens, Helminth / analysis
  • Antigens, Helminth / chemistry
  • Antigens, Helminth / immunology*
  • Blotting, Western / veterinary
  • Cysticercosis / diagnosis
  • Cysticercosis / immunology
  • Cysticercosis / parasitology
  • Cysticercosis / veterinary*
  • Cysticercus / immunology*
  • Cysticercus / isolation & purification
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Helminth Proteins / analysis
  • Helminth Proteins / chemistry
  • Helminth Proteins / immunology*
  • Molecular Weight
  • Sensitivity and Specificity
  • Sheep
  • Sheep Diseases / diagnosis
  • Sheep Diseases / immunology
  • Sheep Diseases / parasitology*

Substances

  • Antigens, Helminth
  • Helminth Proteins