Unnatural amino acid mutagenesis in mapping ion channel function

Curr Opin Neurobiol. 2003 Jun;13(3):264-70. doi: 10.1016/s0959-4388(03)00068-0.


Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure-function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.

Publication types

  • Review

MeSH terms

  • Amino Acids / chemistry*
  • Amino Acids / physiology
  • Animals
  • Chromosome Mapping / methods*
  • Humans
  • Ion Channels / chemistry*
  • Ion Channels / physiology
  • Mutagenesis, Site-Directed / physiology*
  • Oocytes
  • Structure-Activity Relationship
  • Xenopus


  • Amino Acids
  • Ion Channels