Effect of estradiol on chemokine receptor CXCR2 expression in rats: implications for atherosclerosis

Acta Pharmacol Sin. 2003 Jul;24(7):670-4.

Abstract

Aim: To study the effect of 17beta-estradiol on expression of chemokine receptor CXCR2 in monocytes in vivo.

Methods: Expressions of chemokine receptor CXCR2 mRNA and protein were measured by RT-PCR and flow cytometry, respectively.

Results: In both ovary-intact and ovariectomized (OVX) rats, CXCR2 protein and mRNA expression were significantly increased in rats fed with a high-cholesterol diet for 6 weeks. The cholesterol-induced increases in CXCR2 protein and mRNA expression were significantly attenuated in OVX rats injected with estradiol-17beta (17beta-E2) (5 and 20 microg x kg(-1) x d(-1)). In normal diet rats, CXCR2 protein and mRNA expression were increased in OVX rats compared with ovary-intact rats, and this increase was prevented by 17beta-E2.

Conclusion: Both basal and hypercholesterolemia-induced increases in chemokine receptor CXCR2 are modulated by physiological concentrations of estradiol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arteriosclerosis / metabolism
  • Arteriosclerosis / pathology
  • Arteriosclerosis / prevention & control
  • Cell Separation
  • Diet, Atherogenic
  • Estradiol / pharmacology*
  • Estradiol / therapeutic use
  • Female
  • Gene Expression / drug effects*
  • Leukocytes, Mononuclear / metabolism
  • Ovariectomy
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / drug effects
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Interleukin-8B / biosynthesis*
  • Receptors, Interleukin-8B / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Messenger
  • Receptors, Interleukin-8B
  • Estradiol