Inhibitors of HIV-1 reverse transcriptase and fidelity of in vitro DNA replication

J Enzyme Inhib. 1992;6(1):35-46. doi: 10.3109/14756369209041354.


Mechanisms of the effects of the dTTP analogues 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) and 3'-amino-3'-deoxythymidine 5'-triphosphate (NH2 TTP) upon the HIV-1 reverse transcriptase (RT) are discussed. These compounds block the RT in vitro and do so by different kinetic mechanisms. Infidelity of replication is a hallmark of the HIV-1 RT, and replication errors by the enzyme on RNA and DNA templates are discussed. The enzyme's infidelity has ramifications for inhibition: On the one hand, the propensity to produce mutations enhances the ability of the virus to escape inhibitors whereas on the other hand, the infidelity of the reverse transcriptase may allow the development of imaginative inhibitor strategies.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA Replication*
  • Dideoxynucleosides / pharmacology
  • Dideoxynucleotides
  • HIV Reverse Transcriptase
  • HIV-1 / enzymology*
  • Reverse Transcriptase Inhibitors*
  • Thymine Nucleotides / pharmacology*
  • Virus Replication
  • Zidovudine / analogs & derivatives*
  • Zidovudine / pharmacology


  • Dideoxynucleosides
  • Dideoxynucleotides
  • Reverse Transcriptase Inhibitors
  • Thymine Nucleotides
  • Zidovudine
  • 3'-aminothymidine
  • zidovudine triphosphate
  • HIV Reverse Transcriptase