Gene expression analysis in interstitial lung edema induced by saline infusion

Biochim Biophys Acta. 2003 Jul 14;1638(2):149-56. doi: 10.1016/s0925-4439(03)00080-2.


To investigate the molecular events taking place during the development of hydraulic interstitial edema, we analyzed by microarray and conventional molecular techniques the variation of gene expression in lung from rabbits treated with slow-rate saline infusions. This analysis indicates that even a condition characterized by a small increase in extravascular water can have a significant influence on the inflammatory milieu. In this regard, cytokines, in particular TNFalpha, can be considered early mediators capable of inducing secondary effects on the injured tissue. Moreover, two MT1 genes were strongly up-regulated, data consistent with their role as protective molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytokines / metabolism*
  • Gene Expression
  • Infusions, Intravenous
  • Lung Diseases, Interstitial / genetics*
  • Microtubule Proteins / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Pulmonary Edema / chemically induced
  • Pulmonary Edema / genetics*
  • RNA, Messenger / metabolism
  • Rabbits
  • Sodium Chloride


  • Cytokines
  • Microtubule Proteins
  • RNA, Messenger
  • Sodium Chloride