Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections

Clin Diagn Lab Immunol. 2003 Jul;10(4):622-30. doi: 10.1128/cdli.10.4.622-630.2003.


We have found that NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) can be used to differentiate primary and secondary dengue virus infections. This is due to the fact that the NS1-specific IgG antibody cannot be detected before day 9 of illness for primary infection, so the NS1-specific IgG antibodies measured in acute-phase sera must come from previous infection. Comparison of NS1 serotype-specific IgG ELISA with envelope- and membrane-specific capture IgM and IgG ELISA in the differentiation of primary and secondary dengue virus infections showed good correlation (95.90% agreement). Most important, we have found that the serotype of the dengue virus from the majority of patients with primary infection could be correctly identified when convalescent-phase or postinfection sera were analyzed by NS1 serotype-specific IgG ELISA. These findings suggested that NS1 serotype-specific IgG ELISA could be reliably applied for serodiagnosis and seroepidemiological study of dengue virus infection.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Antigens, Viral / immunology*
  • Chlorocebus aethiops
  • Convalescence
  • Dengue / diagnosis*
  • Dengue Virus / classification
  • Dengue Virus / immunology*
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Immunoglobulin G / blood*
  • Immunoglobulin G / immunology
  • Immunoglobulin M / blood*
  • Immunoglobulin M / immunology
  • Serotyping
  • Vero Cells
  • Viral Nonstructural Proteins / immunology*


  • Antibodies, Viral
  • Antigens, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • NS1 protein, dengue-1 virus
  • Viral Nonstructural Proteins