Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila

EMBO J. 2003 Jul 15;22(14):3635-44. doi: 10.1093/emboj/cdg345.

Abstract

Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3'-phosphate 5'-phosphosulfate. Here we report the first molecular identification of a gene that encodes a transmembrane protein required to transport adenosine 3'-phosphate 5'-phosphosulfate from the cytosol into the Golgi lumen. Mutations in this gene, which we call slalom, display defects in Wg and Hh signaling, which are likely due to the lack of sulfation of glycosaminoglycans by the sulfotransferase sulfateless. Analysis of mosaic mutant ovaries shows that sll function is also essential for dorsal-ventral axis determination, suggesting that sll transports the sulfate donor required for sulfotransferase activity of the dorsal-ventral determinant pipe.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Transport
  • Body Patterning / genetics
  • Cytosol / metabolism
  • Drosophila / embryology*
  • Drosophila / genetics*
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism*
  • Female
  • Gene Expression
  • Genes, Insect*
  • Golgi Apparatus / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Ovary / metabolism
  • Phosphoadenosine Phosphosulfate / metabolism*
  • Proteoglycans / metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Sulfate Transporters
  • Sulfates / metabolism
  • Wings, Animal / embryology

Substances

  • Drosophila Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • Proteoglycans
  • Sulfate Transporters
  • Sulfates
  • sll protein, Drosophila
  • Phosphoadenosine Phosphosulfate