Photodestruction intermediates probed by an adjacent reporter molecule

Phys Rev Lett. 2003 Jun 6;90(22):223002. doi: 10.1103/PhysRevLett.90.223002. Epub 2003 Jun 5.


We used a fluorescence resonance energy transfer donor molecule to probe the multiple intermediates in the photoinduced destruction of an acceptor molecule. These intermediates are nonemitting but are still able to quench the fluorescence of the donor at a distance scale shorter than conventional fluorescence resonance energy transfer, suggesting novel biophysical applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biotin / chemistry
  • DNA / chemistry*
  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescent Dyes / chemistry


  • Fluorescent Dyes
  • Biotin
  • DNA