Design of a flow perfusion bioreactor system for bone tissue-engineering applications

Tissue Eng. 2003 Jun;9(3):549-54. doi: 10.1089/107632703322066723.


Several different bioreactors have been investigated for tissue-engineering applications. Among these bioreactors are the spinner flask and the rotating wall vessel reactor. In addition, a new type of culture system has been developed and investigated, the flow perfusion culture bioreactor. Flow perfusion culture offers several advantages, notably the ability to mitigate both external and internal diffusional limitations as well as to apply mechanical stress to the cultured cells. For such investigation, a flow perfusion culture system was designed and built. This design is the outgrowth of important design requirements and incorporates features crucial to successful experimentation with such a system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bioreactors*
  • Bone Substitutes*
  • Mice
  • Osteosarcoma / metabolism
  • Tissue Engineering / instrumentation*
  • Tissue Engineering / methods


  • Bone Substitutes