Antimicrobial peptides in the first line defence of human colon mucosa

Peptides. 2003 Apr;24(4):523-30. doi: 10.1016/s0196-9781(03)00114-1.

Abstract

Antimicrobial peptides and proteins are effector molecules in the protection of epithelial surfaces. We have evaluated the presence of antimicrobial peptides/proteins that can participate in human colonic defence against microbes. A peptide/protein extract of normal human colon mucosa was found to be active against Gram-positive bacteria, Gram-negative bacteria, and fungi. Four polypeptides with antimicrobial activity were isolated from this material and they were identified by N-terminal amino acid sequence analysis as ubiquicidin, histone H2B, eosinophil cationic protein, and phospholipase A(2) (PLA(2)). Using immunodetection and mass spectrometry, LL-37, HNP1-3, and HBD-1 were also identified. Combined, these results indicate that the colon mucosa is protected by a complex mixture of polypeptides, able to kill invading microbes and working in synergy as a barrier against bacterial invasion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / pharmacology*
  • Antimicrobial Cationic Peptides / pharmacology
  • Bacteria / immunology
  • Blood Proteins / chemistry
  • Blotting, Southern
  • Blotting, Western
  • Cathelicidins
  • Chromatography, High Pressure Liquid
  • Colon / pathology*
  • Defensins / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Eosinophil Granule Proteins
  • Histones / chemistry
  • Humans
  • Mass Spectrometry
  • Mucous Membrane / pathology*
  • Peptides / chemistry
  • Peptides / pharmacology
  • Phospholipases A / chemistry
  • Protein Structure, Tertiary
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribonucleases / chemistry
  • Ribosomal Proteins / chemistry
  • Time Factors
  • alpha-Defensins / chemistry
  • beta-Defensins / chemistry

Substances

  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • Blood Proteins
  • Cathelicidins
  • DEFB1 protein, human
  • Defensins
  • Eosinophil Granule Proteins
  • Histones
  • Peptides
  • RNA, Messenger
  • Ribosomal Proteins
  • alpha-Defensins
  • beta-Defensins
  • human neutrophil peptide 1
  • ribosomal protein S30
  • CAP18 lipopolysaccharide-binding protein
  • Ribonucleases
  • Phospholipases A