Characterization of Pellino2, a Substrate of IRAK1 and IRAK4

FEBS Lett. 2003 Jul 17;547(1-3):157-61. doi: 10.1016/s0014-5793(03)00697-5.

Abstract

Interleukin-1 (IL-1) receptor-associated kinases (IRAKs) are central components of Toll/IL-1 receptor (TIR) signaling pathways. In an attempt to discover novel signal transducers in TIR signaling, we identified human Pellino2 as an interaction partner of IRAK4. Pellino2 interacts with kinase-active as well as kinase-inactive IRAK1 and IRAK4. Furthermore, Pellino2 is one of the first substrates identified for IRAK1 and IRAK4. Functional studies using overexpression or RNAi knock-down of Pellino2 suggest a role of Pellino2 as a scaffolding protein similar to Pellino1. However, unlike Pellino1, Pellino2 does not seem to activate a specific transcription factor, but links TIR signaling to basic cellular processes.

MeSH terms

  • Cell Line
  • Cells, Cultured
  • Cloning, Molecular
  • Genes, Reporter
  • Genetic Vectors
  • Humans
  • Interleukin-1 Receptor-Associated Kinases
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism*
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Substrate Specificity
  • Ubiquitin-Protein Ligases

Substances

  • Nuclear Proteins
  • Recombinant Proteins
  • PELI1 protein, human
  • Ubiquitin-Protein Ligases
  • Protein Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • IRAK1 protein, human
  • IRAK4 protein, human
  • Interleukin-1 Receptor-Associated Kinases