Abstract
An effective method to prepare the substrate of alpha-L-fucosidase (AFU) is described. Ethyl 1-thiofucoside with a free 2-OH group was used as the glycosyl donor, and there was found no self-condensed side product. The use of the HF.pyridine reagent to remove the silyl protecting group in the last step afforded a target molecule of high purity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Glucosides / chemical synthesis*
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Glucosides / chemistry
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Glucosides / metabolism*
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Molecular Structure
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Nitro Compounds / chemical synthesis*
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Nitro Compounds / chemistry
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Nitro Compounds / metabolism*
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Substrate Specificity
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alpha-L-Fucosidase / metabolism*
Substances
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2-chloro-4-nitrophenyl alpha-L-fucopyranoside
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Glucosides
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Nitro Compounds
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alpha-L-Fucosidase