Cellulose acetate beads induce release of interleukin-1 receptor antagonist, but not tumour necrosis factor-alpha or interleukin-1beta in human peripheral blood

Inflamm Res. 2003 Jun;52(7):287-90. doi: 10.1007/s00011-003-1173-9.


Objective: Dramatic improvements in clinical symptoms of rheumatoid arthritis and ulcerative colitis were observed after patients received granulocyte and monocyte adsorptive apheresis with a column containing cellulose acetate (CA) beads as adsorptive carriers. This study was to investigate the effect of CA beads on the generation of anti-inflammatory and pro-inflammatory cytokines in human blood.

Materials and methods: We incubated human whole blood with CA beads at 37 degrees C for up to 2 h and measured tumour necrosis factor-alpha (TNF-alpha) interleukin-1beta (IL-1beta) and IL-1 receptor antagonist (IL-1ra) produced by leucocytes. IL-1ra was also measured at the inflow and outflow of a column containing CA beads as leucocyte adsorptive carriers for the treatment of patients with ulcerative colitis.

Results: CA beads induced significant release of IL-1ra from leucocytes, but not TNF-alpha or IL-1beta. In contrast, all three cytokines were released when leucocytes were stimulated with lipopolysaccharide. IL-1ra was also markedly elevated in the outflow of the leucocyte apheresis column.

Conclusions: These results indicate that CA beads selectively induce IL-1ra release from leucocytes which should contribute to the anti-inflammatory effect of granulocyte and monocyte adsorptive apheresis with CA beads as apheresis carriers.

MeSH terms

  • Adolescent
  • Adult
  • Blood Component Removal
  • Cellulose / analogs & derivatives*
  • Cellulose / pharmacology*
  • Colitis, Ulcerative / therapy
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Granulocytes / drug effects
  • Granulocytes / metabolism
  • Humans
  • In Vitro Techniques
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / blood*
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Microspheres
  • Middle Aged
  • Sialoglycoproteins / blood*
  • Tumor Necrosis Factor-alpha / metabolism*


  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Sialoglycoproteins
  • Tumor Necrosis Factor-alpha
  • acetylcellulose
  • Cellulose