Identification of an epitope of SARS-coronavirus nucleocapsid protein

Cell Res. 2003 Jun;13(3):141-5. doi: 10.1038/sj.cr.7290158.

Abstract

The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, it was confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be usefull for the study of SARS-CoV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / blood
  • Antibody Specificity / immunology
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / chemistry
  • Epitopes / immunology*
  • Epitopes / isolation & purification
  • Genetic Vectors / genetics
  • Humans
  • Immunization
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Molecular Sequence Data
  • Nucleocapsid Proteins / chemistry
  • Nucleocapsid Proteins / immunology*
  • Nucleocapsid Proteins / isolation & purification
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology
  • Protein Binding / immunology
  • Rabbits
  • SARS Virus / chemistry
  • SARS Virus / genetics
  • SARS Virus / immunology*

Substances

  • Antibodies, Viral
  • Epitopes
  • Immunoglobulin G
  • Nucleocapsid Proteins
  • Peptide Fragments

Associated data

  • RefSeq/NC_004718