Effects of bisphenol A on the metabolisms of active oxygen species in mouse tissues

Environ Res. 2003 Sep;93(1):31-5. doi: 10.1016/s0013-9351(03)00062-8.


We investigated the modifications in endogenous antioxidant capacity, including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, oxidative stress index, reduced glutathione (GSH), glutathione disulfide (GSSG), and thiobarbituric acid-reactive substance (TBARS) in the brain, liver, kidney, and testes of mice under bisphenol A (BPA), an endocrine disrupter, treated for 5 days. BPA was administrated intraperitoneally at doses of 25 and 50mg/kg/day. The TBARS levels were not affected by BPA administrations. The SOD activities increased and the catalase activities decreased in the liver after BPA administration. The GPx activity decreased in the kidney. The levels of GSH+GSSG increased in the brain, kidney, liver, and testes, while, the levels of GSH decreased in the testes. SOD converts superoxide into hydrogen peroxide, and catalase and GPx convert hydrogen peroxide into hydrogen oxide. Our results suggest that the injection of BPA induces overproduction of hydrogen peroxide in the mouse organs. Hydrogen peroxide is easily converted to hydroxy radical. The decrease of GSH and the increase of GSSG may be caused by the hydroxy radical. BPA may show its toxicity by increasing hydrogen peroxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzhydryl Compounds
  • Brain / metabolism
  • Catalase / metabolism
  • Estrogens, Non-Steroidal / metabolism
  • Estrogens, Non-Steroidal / toxicity*
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Kidney / metabolism
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • Phenols / metabolism
  • Phenols / toxicity*
  • Reactive Oxygen Species / metabolism*
  • Superoxide Dismutase / metabolism
  • Testis / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism


  • Benzhydryl Compounds
  • Estrogens, Non-Steroidal
  • Phenols
  • Reactive Oxygen Species
  • Thiobarbituric Acid Reactive Substances
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione
  • bisphenol A