Analysis of RNA-protein interactions by flow cytometry

Curr Opin Mol Ther. 2003 Jun;5(3):235-40.


Flow cytometry, in combination with advances in bead coding technologies, is maturing as a powerful high-throughput approach for analyzing molecular interactions. Applications of this technology include antibody assays and single nucleotide polymorphism mapping. This review describes the recent development of a microbead flow cytometric approach to analyze RNA-protein interactions and discusses emerging bead coding strategies that together will allow genome-wide identification of RNA-protein complexes. The microbead flow cytometric approach is flexible and provides new opportunities for functional genomic studies and small-molecule screening.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Flow Cytometry / methods*
  • Genomics
  • Humans
  • Microspheres
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides / metabolism
  • Proteins / metabolism*
  • RNA / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Surface Properties


  • Oligonucleotides
  • Proteins
  • Recombinant Fusion Proteins
  • RNA