Coexpression of matrilysin and laminin-5 gamma2 chain may contribute to tumor cell migration in colorectal carcinomas

Dig Dis Sci. 2003 Jul;48(7):1262-7. doi: 10.1023/a:1024142722640.

Abstract

We attempted to examine the correlation between matrilysin and laminin-5 gamma2 chain expression with reference to the number of dedifferentiation units along the entire invasive front (tumor budding). Immunostaining for hMMP-7 and laminin-5 gamma2 chain was performed in 50 T1 colorectal carcinomas, and immunoreactivity was evaluated at the invasive front of the tumor. On hematoxylin-eosin sections, the number of tumor budding was counted. The localization of matrilysin tended to be widespread compared with that of laminin-5 gamma2 chain. Matrilysin and laminin-5 gamma2 chain expression were positive in 28 (56%) and 15 (30%) tumors respectively. There was a significant correlation between matrilysin and laminin-5 gamma2 chain expression (P = 0.02). Matrilysin(+)/laminin-5 gamma2 chain(+) tumors had a significantly greater amount of tumor budding than matrilysin(-)/laminin-5 gamma2 chain(-) tumors (P = 0.003) or matrilysin(+)/laminin-5 gamma2 chain(-) tumors (P = 0.03). In conclusions, coexpression of matrilysin and laminin-5 gamma2 chain may contribute to tumor cell migration in colorectal carcinomas.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Adhesion Molecules / biosynthesis*
  • Cell Movement / physiology*
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / pathology*
  • Female
  • Humans
  • Immunohistochemistry
  • Laminin / biosynthesis*
  • Male
  • Matrix Metalloproteinase 7 / biosynthesis*
  • Matrix Metalloproteinases / metabolism
  • Middle Aged
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Tumor Cells, Cultured

Substances

  • Cell Adhesion Molecules
  • LAMC2 protein, human
  • Laminin
  • kalinin
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase 7