An influence of ABO blood group on the rate of proteolysis of von Willebrand factor by ADAMTS13

J Thromb Haemost. 2003 Jan;1(1):33-40. doi: 10.1046/j.1538-7836.2003.00007.x.


The susceptibility of von Willebrand factor (VWF) of blood group O, A, B and AB to proteolysis by the ADAMTS13 metalloprotease was investigated. Multimeric analysis indicated that the rate of VWF proteolysis differed between blood groups and was greater for group O VWF than for non-O VWF in the rank order O >/= B > A >/= AB. Measurement of the collagen binding activity of VWF of each blood group following proteolysis for a fixed time interval corroborated the results obtained on multimer analysis: the loss of collagen binding activity was greater for VWF of group O compared with non-O VWF, in the rank order O >/= B > A >/= AB. Ristocetin was found to increase the rate of VWF proteolysis approximately two-fold; the differential between blood groups was retained in the presence of ristocetin.

Publication types

  • Comparative Study

MeSH terms

  • ABO Blood-Group System / metabolism*
  • ADAM Proteins
  • ADAMTS13 Protein
  • Blood Donors
  • Collagen / metabolism
  • Densitometry / methods
  • Humans
  • Metalloendopeptidases / metabolism*
  • Ristocetin / pharmacology
  • Statistics as Topic
  • Time Factors
  • von Willebrand Factor / analysis
  • von Willebrand Factor / metabolism*


  • ABO Blood-Group System
  • von Willebrand Factor
  • Ristocetin
  • Collagen
  • ADAM Proteins
  • Metalloendopeptidases
  • ADAMTS13 Protein
  • ADAMTS13 protein, human