Patched 1 conditional null allele in mice

Genesis. 2003 Jul;36(3):158-61. doi: 10.1002/gene.10208.

Abstract

The patched gene (Ptc) is a member of the hedgehog signaling pathway which plays a central role in the development of many invertebrate and vertebrate tissues. In addition, Ptc and a number of other pathway members are mutated in some common human cancers. Patched is the receptor for the hedgehog ligand and in the mouse ablation of the Ptc gene leads to developmental defects and an embryonic lethal phenotype. Here we describe a conditional Ptc allele in mice which will have utility for the temporospatial ablation of Ptc function.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Animals
  • Blotting, Southern
  • DNA Primers
  • Gene Silencing*
  • Gene Targeting
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / deficiency*
  • Membrane Proteins / genetics*
  • Mice / genetics*
  • Patched Receptors
  • Patched-1 Receptor
  • Phenotype
  • Polymerase Chain Reaction
  • Receptors, Cell Surface

Substances

  • DNA Primers
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • PTCH protein, human
  • Patched Receptors
  • Patched-1 Receptor
  • Ptch1 protein, mouse
  • Receptors, Cell Surface