Prediction of liver histological lesions with biochemical markers in patients with chronic hepatitis B

J Hepatol. 2003 Aug;39(2):222-30. doi: 10.1016/s0168-8278(03)00171-5.


Background aims: Liver biopsy is the gold standard for assessing hepatitis B virus (HBV)-related histology. The aim was to determine the diagnostic utility of noninvasive serum markers in patients with chronic hepatitis B.

Methods: The aminotransferases and indices including alpha(2)-macroglobulin, apolipoprotein A1, haptoglobin, gamma-glutamyl-transpeptidase (GGT), and total bilirubin (Fibrotest), and ALT (Actitest) were compared with liver histology. The primary outcomes were A2-A3 activity and F2-F4 fibrosis (METAVIR).

Results: Two hundred and nine patients were included. Forty-one patients (20%) had A2-A3 activity and 61 (29%) had F2-F4 fibrosis. AST and GGT (P<0.001) were independently associated with A2-A3 activity. AST, ALT, and Actitest accurately predicted activity ((areas under receiver operating characteristic (ROC) curves (AUROC), 0.81-0.82+/-0.04)); an AST or ALT< or =30IU/l excluded significant activity with 96% certainty. Fibrotest accurately predicted F2-F4 fibrosis (AUROC, 0.78+/-0.04). Fibrotest scores (range, 0-1.0) < or =0.20 and >0.80 had negative and positive predictive values of 92%, respectively. Restricting biopsy to patients with intermediate scores (>0.20 and < or =0.80) may prevent liver biopsies in 46% of patients while maintaining 92% accuracy.

Conclusions: The aminotransferases and an index including five biochemical markers are accurate noninvasive markers of HBV-related activity and fibrosis, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoproteins A / blood
  • Biomarkers
  • Biopsy
  • Female
  • Fibrosis
  • Haptoglobins / metabolism
  • Hepatitis B, Chronic / blood*
  • Hepatitis B, Chronic / pathology*
  • Humans
  • Liver / pathology*
  • Male
  • Middle Aged
  • Predictive Value of Tests
  • Prospective Studies
  • Retrospective Studies
  • Transaminases / blood
  • alpha-Macroglobulins / metabolism


  • Apolipoproteins A
  • Biomarkers
  • Haptoglobins
  • alpha-Macroglobulins
  • Transaminases