Abstract
Here we clone the Xenopus 5-HT2B receptor cDNA and describe its spatio-temporal mRNA expression within the developing larval brain and visual system. Expression of the 5-HT2B transcripts is compared to that of 5-HT2C as well as proliferation and neurogenic markers. In developing brain and retina, 5-HT2B and 2C mRNAs are mainly expressed in proliferative regions. We suggest that these receptors may play a role in the larval secondary neurogenesis by mediating mitogenic effects of serotonin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain / embryology
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Brain / metabolism*
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Bromodeoxyuridine / pharmacokinetics
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Cloning, Molecular
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Cyclin D1 / genetics
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Cyclin D1 / metabolism
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Embryo, Nonmammalian
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Eye / embryology
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Eye / metabolism*
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Gene Expression Regulation, Developmental*
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Genetic Markers
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In Situ Hybridization
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Larva / genetics
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Larva / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Molecular Sequence Data
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RNA, Messenger / biosynthesis
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Radiation-Sensitizing Agents / pharmacokinetics
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Receptor, Notch1
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Receptor, Serotonin, 5-HT2A
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Receptor, Serotonin, 5-HT2B
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Receptors, Cell Surface*
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Receptors, Serotonin / genetics
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Receptors, Serotonin / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Transcription Factors*
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Xenopus / genetics
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Xenopus Proteins / metabolism
Substances
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Genetic Markers
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Membrane Proteins
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RNA, Messenger
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Radiation-Sensitizing Agents
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Receptor, Notch1
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Receptor, Serotonin, 5-HT2A
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Receptor, Serotonin, 5-HT2B
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Receptors, Cell Surface
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Receptors, Serotonin
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Transcription Factors
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Xenopus Proteins
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Cyclin D1
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Bromodeoxyuridine