Expression profiles of the novel human connexin genes hCx30.2, hCx40.1, and hCx62 differ from their putative mouse orthologues

Cell Commun Adhes. Jan-Feb 2003;10(1):27-36. doi: 10.1080/15419060302063.

Abstract

In this study we show by Northern blot hybridization that the novel human (h) connexin (Cx) genes hCx25, hCx30.2, hCx31.9, hCx40.1, hCx59, and hCx62 are transcribed in different adult tissues. The hCx25 RNA is slightly expressed in placenta, and hCx59 and hCx62 RNA are both transcribed in skeletal muscle, although the latter is also slightly expressed in heart. Expression profiles of three orthologous human (h) and mouse (m) connexin gene pairs, i.e., hCx30.2 versus mCx29, hCx40.1 versus mCx39, and hCx62 versus mCx57, differ strongly, in contrast to other orthologous connexins with higher sequence identities. Thus, several of the new human connexin genes appear to have evolved to different expression patterns and presumably to different functions compared to their orthologues in the mouse genome. (121)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Connexins / biosynthesis*
  • Connexins / chemistry
  • Exons
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muscle, Skeletal / metabolism
  • Myocardium / metabolism
  • Placenta / metabolism
  • Polymerase Chain Reaction
  • RNA / chemistry
  • RNA, Messenger / metabolism
  • Sequence Homology, Nucleic Acid
  • Tissue Distribution
  • Transcription, Genetic

Substances

  • Connexins
  • GJA10 protein, human
  • GJD4 protein, human
  • RNA, Messenger
  • RNA