Abstract
Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 microM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 microM) further increased cis-cytokinins, up to 2.2 nM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenine / analogs & derivatives*
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Adenine / pharmacology*
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Alginates
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Benzyl Compounds
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Cell Survival
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Cells, Cultured
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Cells, Immobilized / drug effects
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Cells, Immobilized / metabolism
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Cyclin-Dependent Kinases / antagonists & inhibitors
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Cytokinins / biosynthesis
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Glucuronic Acid
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Hexuronic Acids
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Isopentenyladenosine / biosynthesis*
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Kinetin
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Nicotiana / drug effects*
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Nicotiana / metabolism*
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Purines / pharmacology*
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Reproducibility of Results
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Roscovitine
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Sensitivity and Specificity
Substances
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Alginates
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Benzyl Compounds
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Cytokinins
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Hexuronic Acids
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Purines
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Roscovitine
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Isopentenyladenosine
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Glucuronic Acid
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Cyclin-Dependent Kinases
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Adenine
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benzylaminopurine
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Kinetin