Abstract
In Caenorhabditis elegans, the forkhead protein DAF-16 transduces insulin-like signals that regulate larval development and adult lifespan. To identify DAF-16-dependent transcriptional alterations that occur in a long-lived C. elegans strain, we used cDNA microarrays and genomic analysis to identify putative direct and indirect DAF-16 transcriptional target genes. Our analysis suggests that DAF-16 action regulates a wide range of physiological responses by altering the expression of genes involved in metabolism, energy generation and cellular stress responses. Furthermore, we observed a large overlap between DAF-16-dependent transcription and genes normally expressed in the long-lived dauer larval stage. Finally, we examined the in vivo role of 35 of these target genes by RNA-mediated interference and identified one gene encoding a putative protease that is necessary for the daf-2 Age phenotype.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans Proteins / biosynthesis
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / physiology*
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DNA, Helminth / genetics
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Forkhead Transcription Factors
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Gene Expression Profiling*
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Gene Expression Regulation, Developmental*
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Larva
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Longevity / genetics*
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Oligonucleotide Array Sequence Analysis
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Phenotype
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Promoter Regions, Genetic / genetics
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RNA Interference
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RNA, Helminth / biosynthesis
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RNA, Helminth / genetics
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Regulatory Sequences, Nucleic Acid
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Transcription Factors / physiology*
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Transcription, Genetic*
Substances
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Caenorhabditis elegans Proteins
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DNA, Helminth
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Forkhead Transcription Factors
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RNA, Helminth
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RNA, Messenger
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Transcription Factors
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daf-16 protein, C elegans