Transcriptional outputs of the Caenorhabditis elegans forkhead protein DAF-16

Aging Cell. 2003 Apr;2(2):111-21. doi: 10.1046/j.1474-9728.2003.00043.x.


In Caenorhabditis elegans, the forkhead protein DAF-16 transduces insulin-like signals that regulate larval development and adult lifespan. To identify DAF-16-dependent transcriptional alterations that occur in a long-lived C. elegans strain, we used cDNA microarrays and genomic analysis to identify putative direct and indirect DAF-16 transcriptional target genes. Our analysis suggests that DAF-16 action regulates a wide range of physiological responses by altering the expression of genes involved in metabolism, energy generation and cellular stress responses. Furthermore, we observed a large overlap between DAF-16-dependent transcription and genes normally expressed in the long-lived dauer larval stage. Finally, we examined the in vivo role of 35 of these target genes by RNA-mediated interference and identified one gene encoding a putative protease that is necessary for the daf-2 Age phenotype.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans Proteins / biosynthesis
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • DNA, Helminth / genetics
  • Forkhead Transcription Factors
  • Gene Expression Profiling*
  • Gene Expression Regulation, Developmental*
  • Larva
  • Longevity / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Promoter Regions, Genetic / genetics
  • RNA Interference
  • RNA, Helminth / biosynthesis
  • RNA, Helminth / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid
  • Transcription Factors / physiology*
  • Transcription, Genetic*


  • Caenorhabditis elegans Proteins
  • DNA, Helminth
  • Forkhead Transcription Factors
  • RNA, Helminth
  • RNA, Messenger
  • Transcription Factors
  • daf-16 protein, C elegans