Measurement of retinoids and beta-carotene 15,15'-dioxygenase activity in HR-1 hairless mouse skin with UV exposure

J Nutr Sci Vitaminol (Tokyo). 2003 Feb;49(1):69-72. doi: 10.3177/jnsv.49.69.


We investigated the vitamin A status and beta-carotene 15,15'-dioxygenase activity in hairless mice with UV exposure to assess the regulation of vitamin A metabolism after UV irradiation. HR-1 hairless mice were irradiated with UV at 3 J/cm2 for 5 d. After UV irradiation, the mice were sacrificed and samples were obtained to analyze the retinoid concentration, expression of RXR-alpha, and beta-carotene 15,15'-dioxygenase activity. After UV exposure, the skin retinoid concentration was significantly lower as well as the expression of RXR-alpha. Higher skin beta-carotene dioxygenase activity was observed in the UV group as compared to the control group. We found no significant differences in the alpha-tocopherol concentration or acrolein levels in the skins of the two groups. In conclusion, the elevation of beta-carotene 15,15'-dioxygenase activity in hairless mice after UV exposure may be a response to reduction of the skin retinoid concentration.

MeSH terms

  • Animals
  • Blotting, Western
  • Liver / chemistry
  • Male
  • Mice
  • Mice, Hairless
  • Oxygenases / metabolism*
  • Receptors, Retinoic Acid / analysis
  • Retinoid X Receptors
  • Retinoids / analysis*
  • Retinoids / blood
  • Skin / chemistry
  • Skin / enzymology*
  • Skin / radiation effects*
  • Transcription Factors / analysis
  • Ultraviolet Rays*
  • alpha-Tocopherol / analysis
  • beta-Carotene 15,15'-Monooxygenase


  • Receptors, Retinoic Acid
  • Retinoid X Receptors
  • Retinoids
  • Transcription Factors
  • Oxygenases
  • Bco1 protein, mouse
  • beta-Carotene 15,15'-Monooxygenase
  • alpha-Tocopherol