Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

Biotechnol Lett. 2003 Feb;25(4):321-5. doi: 10.1023/a:1022339929026.


A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adjuvants, Immunologic / analysis
  • Animals
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods*
  • Cells, Cultured
  • Electrochemistry / instrumentation
  • Electrochemistry / methods
  • Equipment Design
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase / metabolism
  • Nitric Oxide Synthase Type II
  • Nitrous Oxide / metabolism
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Toxicity Tests / instrumentation*
  • Toxicity Tests / methods


  • Adjuvants, Immunologic
  • Lipopolysaccharides
  • Nitric Oxide
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Nitrous Oxide