Genomovar diversity amongst Burkholderia cepacia complex isolates from an Australian adult cystic fibrosis unit

Eur J Clin Microbiol Infect Dis. 2003 Jul;22(7):434-7. doi: 10.1007/s10096-003-0949-8. Epub 2003 Jun 27.

Abstract

In this study, a combination of recA-based PCR assays and 16S rDNA restriction fragment length polymorphism (RFLP) analysis was used to determine the genomovar diversity of clinical Burkholderia cepacia complex isolates. Twenty-eight isolates were prospectively collected from patients attending a large Australian adult cystic fibrosis (CF) unit, 22 isolates were referred from other Australian CF units and a further eight isolates originated from patients without CF. The 28 prospectively collected isolates were distributed amongst the following genomovars: Burkholderia cepacia genomovar I (28.6%), Burkholderia multivorans (21.4%), Burkholderia cepacia genomovar III (39.3%), Burkholderia vietnamiensis(3.6%) and Burkholderia ambifaria (7.1%). The results of this study highlight the usefulness of 16S rDNA RFLP typing for the identification of other Burkholderia spp. and non-fermenting gram-negative bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Australia / epidemiology
  • Burkholderia Infections / microbiology
  • Burkholderia cepacia / classification
  • Burkholderia cepacia / genetics
  • Burkholderia cepacia / isolation & purification*
  • Cystic Fibrosis / complications
  • Cystic Fibrosis / microbiology*
  • DNA, Bacterial / genetics
  • Genetic Variation
  • Humans
  • Polymerase Chain Reaction
  • Prospective Studies
  • Respiratory Tract Infections / microbiology

Substances

  • DNA, Bacterial