Synthesis and properties of phosphorylated 3'-O-beta-D-ribofuranosyl-2'-deoxythymidine

Nucleosides Nucleotides Nucleic Acids. 2003 Apr;22(4):359-71. doi: 10.1081/NCN-120022028.

Abstract

A strategy was developed for the synthesis of 3'-O-beta-D-ribofuranosyl 2'-deoxythymidine derivatives using three different protecting groups, which allows the synthesis of a phosphoramidite building block for oligonucleotide synthesis. Likewise the 5'-O- and 5''-O-phosphorylated analogues were synthesized and their conformation was determined using NMR spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry*
  • Organophosphorus Compounds / chemistry
  • Phosphorylation
  • Thymidine / chemical synthesis*
  • Thymidine / chemistry*

Substances

  • Oligonucleotides
  • Organophosphorus Compounds
  • phosphoramidite
  • Thymidine