Differential expression of sphingolipids in P-glycoprotein or multidrug resistance-related protein 1 expressing human neuroblastoma cell lines

FEBS Lett. 2003 Jul 31;548(1-3):28-32. doi: 10.1016/s0014-5793(03)00721-x.

Abstract

The sphingolipid composition and multidrug resistance status of three human neuroblastoma cell lines were established. SK-N-FI cells displayed high expression and functional (efflux) activity of P-glycoprotein, while multidrug resistance-related protein 1 was relatively abundant and most active in SK-N-AS cells. These two cell lines exhibited higher sphingolipid levels, compared to SK-N-DZ, which had the lowest activity of either ATP-binding cassette transporter protein. SK-N-DZ cells also differed in ganglioside composition with predominant expression of b-series gangliosides. In conclusion, these three neuroblastoma cell lines offer a good model system to study sphingolipid metabolism in relation to ATP-binding cassette transporter protein function.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / physiology
  • Antineoplastic Agents / pharmacokinetics
  • Drug Resistance, Neoplasm
  • Gangliosides / analysis
  • Humans
  • Multidrug Resistance-Associated Proteins / metabolism*
  • Multidrug Resistance-Associated Proteins / physiology
  • Neuroblastoma / pathology*
  • Sphingolipids / biosynthesis*
  • Tumor Cells, Cultured

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antineoplastic Agents
  • Gangliosides
  • Multidrug Resistance-Associated Proteins
  • Sphingolipids
  • multidrug resistance-associated protein 1