Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7

Carbohydr Res. 2003 Aug 12;338(17):1797-800. doi: 10.1016/s0008-6215(03)00272-6.

Abstract

A range of synthetic methyl hexagalacturonates were used as potential hapten inhibitors in competitive-inhibition enzyme-linked immunosorbent assays (ELISAs) with anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. The selective inhibition of these antibodies by different haptens provides insight into the structures of the partially methyl-esterified pectin epitopes of these widely used monoclonal antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Binding, Competitive
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / chemistry
  • Haptens / chemistry
  • Haptens / pharmacology
  • Methylation
  • Pectins / chemical synthesis*
  • Pectins / immunology
  • Pectins / pharmacology

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Haptens
  • Pectins
  • polygalacturonic acid