From genotype to phenotype: correlating XRCC1 polymorphisms with mutagen sensitivity

DNA Repair (Amst). 2003 Aug 12;2(8):901-8. doi: 10.1016/s1568-7864(03)00085-5.

Abstract

This study correlated the extent of induced in vitro chromosomal damage, assessed by the mutagen sensitivity assay, with genotypes of the X-ray repair cross complementing group 1 (XRCC1) gene, which encodes for a base excision repair protein. There are two common polymorphisms that cause amino acid substitutions in XRCC1, one at codon 194 in exon 6 and another at codon 399 in exon 10. We genotyped these two polymorphisms in 524 healthy subjects and performed mutagen sensitivity assays using both bleomycin and benzo[a]pyrene-diol-epoxide (BPDE) as challenge mutagens. Our results showed that individuals with the wildtype exon 6 Arg/Arg exhibited significantly higher values of chromosomal breaks per cell (b/c) than those with one or two variant Trp alleles (P=0.005 for bleomycin and P=0.05 for BPDE). For the exon 10 polymorphism, subjects who were Gln/Gln homozygotes had higher b/c than did those with other genotypes, with evidence of a gene dosage effect. When we combined the two polymorphic sites and used the exon 6 Arg/Trp and Trp/Trp and exon 10 Arg/Arg genotypes as the reference category, these differences were enhanced for bleomycin sensitivity (P for trend = 0.032), but not for BPDE sensitivity (P for trend = 0.821). These data are biologically plausible since codon 399 is located within the BRCA1 C-terminus functional domain and codon 194 is in the linker region of the XRCC1 N-terminal functional domain. To our knowledge, this is the largest study conducted evaluating the functional relevance of these polymorphisms.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • Adult
  • Bleomycin
  • DNA Primers
  • DNA Repair / genetics*
  • DNA-Binding Proteins / genetics*
  • European Continental Ancestry Group
  • Exons
  • Female
  • Humans
  • Linkage Disequilibrium
  • Male
  • Mutagenicity Tests
  • Phenotype*
  • Polymorphism, Genetic*
  • Smoking / genetics
  • X-ray Repair Cross Complementing Protein 1

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human
  • Bleomycin
  • 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide