Measurement of histone acetyltransferase and histone deacetylase activities and kinetics of histone acetylation

Methods. 2003 Sep;31(1):12-23. doi: 10.1016/s1046-2023(03)00083-5.

Abstract

Dynamic histone acetylation has a role in chromatin remodeling and in the regulation of transcription. Histone deacetylases (HDACs) and histone acetyltransferases (HATs) catalyze reversible histone acetylation. HATs and HDACs exist as multiprotein complexes that have coactivator and corepressor activities, respectively. The steady-state level of acetylation at a chromatin site is determined by the local net activities of these enzymes. Here we describe methods to isolate different subcellular fractions (cytosol, nuclei, tightly bound nuclear, loosely bound nuclear, immunoprecipitated multiprotein complexes, and nuclear matrix) to determine the subcellular distribution of HAT and HDAC activities. Procedures to assay the activities of these enzymes and to measure the kinetics of histone acetylation and deacetylation are presented.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylation
  • Acetyltransferases / isolation & purification*
  • Acetyltransferases / metabolism*
  • Animals
  • Cell Fractionation / methods
  • Cell Nucleus / enzymology
  • Cells, Cultured
  • Chickens
  • Chromatography / methods
  • Dimerization
  • Durapatite
  • Erythrocytes / enzymology
  • Histone Acetyltransferases
  • Histone Deacetylases / isolation & purification*
  • Histone Deacetylases / metabolism*
  • Histones / metabolism*
  • Kinetics
  • Mammals

Substances

  • Histones
  • Durapatite
  • Acetyltransferases
  • Histone Acetyltransferases
  • Histone Deacetylases