A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development

Science. 2004 Mar 26;303(5666):2022-5. doi: 10.1126/science.1088060. Epub 2003 Jul 31.

Abstract

Plant microRNAs (miRNAs) show a high degree of sequence complementarity to, and are believed to guide the cleavage of, their target messenger RNAs. Here, I show that miRNA172, which can base-pair with the messenger RNA of a floral homeotic gene, APETALA2, regulates APETALA2 expression primarily through translational inhibition. Elevated miRNA172 accumulation results in floral organ identity defects similar to those in loss-of-function apetala2 mutants. Elevated levels of mutant APETALA2 RNA with disrupted miRNA172 base pairing, but not wild-type APETALA2 RNA, result in elevated levels of APETALA2 protein and severe floral patterning defects. Therefore, miRNA172 likely acts in cell-fate specification as a translational repressor of APETALA2 in Arabidopsis flower development.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antisense Elements (Genetics)
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Arabidopsis Proteins / physiology
  • Base Pairing
  • Binding Sites
  • Flowers / anatomy & histology
  • Flowers / growth & development*
  • Gene Expression Regulation, Plant*
  • Genes, Homeobox
  • Genes, Plant
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • In Situ Hybridization
  • MicroRNAs / chemistry
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Mutation
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Phenotype
  • Plant Proteins*
  • Plants, Genetically Modified
  • Protein Biosynthesis*
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • RNA, Plant / chemistry
  • RNA, Plant / genetics

Substances

  • APETALA2 protein, Arabidopsis
  • Antisense Elements (Genetics)
  • Arabidopsis Proteins
  • HEN1 protein, Arabidopsis
  • Homeodomain Proteins
  • MicroRNAs
  • Nuclear Proteins
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant