Evaluation of the clinical and allergen specific serum immunoglobulin E responses to oral challenge with cornstarch, corn, soy and a soy hydrolysate diet in dogs with spontaneous food allergy

Vet Dermatol. 2003 Aug;14(4):181-7. doi: 10.1046/j.1365-3164.2003.00338.x.


Fourteen dogs with known clinical hypersensitivity to soy and corn were maintained on a limited antigen duck and rice diet until cutaneous manifestations of pruritus were minimal (78 days). Sequential oral challenges with cornstarch, corn and soy were then performed. Subsequently, the dogs were fed a diet containing hydrolysed soy protein and cornstarch. Throughout the study period the dogs were examined for cutaneous manifestations of pruritus and, additionally, serum was collected for measurement of allergen-specific and total immunoglobulin (Ig)E concentrations. Intradermal testing with food antigens was performed prior to entry into the study and after 83 days. A statistically significant clinical improvement was measured between days 0 and 83. Significant pruritus was induced after oral challenge with cornstarch, corn and soy (P = 0.04, 0.002, 0.01, respectively) but not with the hydrolysed diet (P = 0.5). The positive predictive value of the skin test for soy and corn allergy was reduced after feeding a soy and corn free diet. Although increases in soy and corn-specific serum IgE concentrations were measured in individual dogs post challenge they were not statistically significant and could not be used to predict clinical hypersensitivity.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed
  • Animals
  • Antigens
  • Diet*
  • Dog Diseases / blood
  • Dog Diseases / diagnosis*
  • Dog Diseases / immunology*
  • Dogs
  • Female
  • Food Hypersensitivity / diagnosis
  • Food Hypersensitivity / immunology
  • Food Hypersensitivity / veterinary*
  • Immunoglobulin E / blood*
  • Immunoglobulin E / immunology
  • Intradermal Tests / standards
  • Intradermal Tests / veterinary*
  • Male
  • Predictive Value of Tests
  • Soybean Proteins / immunology
  • Soybeans / immunology
  • Zea mays / immunology


  • Antigens
  • Soybean Proteins
  • Immunoglobulin E