Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: Using Dp2 peptide and fungal immunomodulatory peptide

J Allergy Clin Immunol. 2003 Aug;112(2):301-10. doi: 10.1067/mai.2003.1619.


Background: Local nasal immunotherapy (LNIT) is an effective immunotherapy. Peptides derived from the group 2 allergen of Dermatophagoides pteronyssinus, Dp2 28-40 and Dp2 28-40A, and fungal immunomodulatory peptide (FIP) have been shown to act as T(H)1 potential and response-inducing adjuvant. LNIT by the use of Dp2 peptides in conjunction with FIP were investigated.

Objective: We sought to determine whether Dp2-induced airway inflammation in mice could be downregulated by Dp2 peptides or a mixture of Dp2 peptides with FIP.

Method: Mice were sensitized with rDp2 followed by LNIT with Dp2 peptides, FIP, or FIP and a mixture of Dp2 peptides. After intratracheal challenge with rDp2, the airway inflammation and hyperresponsiveness were determined by bronchoalveolar lavage fluid (BALF) analysis and methacholine challenge.

Results: Both Dp2 peptides and FIP were able to inhibit rDp2-induced airway inflammation and airway hyperresponsiveness. An increase in IFN-gamma and a decrease in IL-5 in BALF and sera were found after LNIT with Dp2 peptides, FIP, and mixtures of both. Serum levels of TGF-beta were reduced after LNIT with FIP and Dp2 28-40. Penh values were significantly decreased after methacholine challenge in both the early and late phase.

Conclusions: LNIT with allergen-derived peptides and FIP can produce an anti-inflammatory effect on allergen-induced airway inflammation. LNIT with selected peptides and FIP might be a good alternative therapy for allergic airway disease.

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antigens, Dermatophagoides / administration & dosage
  • Antigens, Dermatophagoides / chemistry
  • Antigens, Dermatophagoides / metabolism*
  • Arthropod Proteins
  • Blood Cells / drug effects
  • Blood Cells / metabolism
  • Bronchitis / drug therapy*
  • Bronchitis / etiology*
  • Bronchitis / pathology
  • Bronchitis / physiopathology
  • Cytokines / biosynthesis
  • Cytokines / blood
  • Fungal Proteins / administration & dosage
  • Immunoglobulins / metabolism
  • Immunotherapy* / methods
  • Lectins / administration & dosage
  • Leukocyte Count
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Peptide Fragments / administration & dosage
  • Respiratory Function Tests


  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Cytokines
  • Dermatophagoides pteronyssinus antigen p 2
  • Fungal Proteins
  • Immunoglobulins
  • Lectins
  • Peptide Fragments