[Cellulose decomposition under nitrogen deficiency by bacteria isolated from the intestines of phytophagous vertebrates]

N A Ushakova; L P Belov; A A Varshavskiĭ; A A Kozlova; T V Kolganova; E S Bulygina; T P Turova

[Cellulose decomposition under nitrogen deficiency by bacteria isolated from the intestines of phytophagous vertebrates]

Mikrobiologiia. 2003 May-Jun;72(3):400-6.

[Article in Russian]

Authors

N A Ushakova¹, L P Belov, A A Varshavskiĭ, A A Kozlova, T V Kolganova, E S Bulygina, T P Turova

Affiliation

¹ Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii pr.33, Moscow, 119071 Russia.

PMID: 12901017

Abstract

A nitrogen-fixing strain identified as Klebsiella pneumonia 402-2 and two endoglucanase-synthesizing Bacillus strains were isolated from the intestines of phytophagous animals. One of the Bacillus strains was identified as Bacillus subtilis GL. Klebsiella pneumoniae 402-2 increased the endoglucanase activity of both Bacillus strains in mixed cultures. The data on the taxonomic position of strains 402-2 and GL and on the nitrogen-fixing capacity of strain 402-2 were confirmed by sequencing and analyzing their 16S rRNA genes and by amplifying the nitrogenase gene nifH.

MeSH terms

Animals
Arvicolinae / microbiology*
Bacillus / enzymology*
Bacillus / genetics
Bacillus / isolation & purification
Bacillus subtilis / classification
Bacillus subtilis / enzymology
Bacillus subtilis / genetics
Birds / microbiology*
Cellulase / metabolism*
Cellulose / metabolism*
Coculture Techniques
Intestine, Large / microbiology
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification
Klebsiella pneumoniae / metabolism*
Molecular Sequence Data
Nitrogen / metabolism
Oxidoreductases / metabolism
Phylogeny
RNA, Bacterial / analysis
RNA, Ribosomal, 16S / analysis
Sequence Analysis

Substances

RNA, Bacterial
RNA, Ribosomal, 16S
Cellulose
Oxidoreductases
nitrogenase reductase
Cellulase
Nitrogen

Associated data

GENBANK/AY114158
GENBANK/AY114159