A simple and rapid method to determine the zygosity of uPA-transgenic SCID mice

Biochem Biophys Res Commun. 2003 Aug 22;308(2):375-8. doi: 10.1016/s0006-291x(03)01388-3.


Successful transplantation of xenogeneic hepatocytes into uPA-transgenic SCID mice depends on the zygosity of the recipient mice. Normally, the difference between homozygous and heterozygous animals is determined via a quantitative Southern blot. We sequenced a part of the mouse genome that is eliminated upon integration of the transgene in the genome. Based on that sequence we developed a multiplex PCR that allows the unambiguous discrimination of negative, heterozygous, and homozygous uPA-transgenic SCID mice in a single day procedure. The speed of the procedure is an essential quality because transplantation of xenogeneic hepatocytes into uPA-SCID mice should be done as soon as possible after birth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Base Sequence
  • Disease Models, Animal
  • Hepatitis B / etiology
  • Hepatitis C / etiology
  • Hepatocytes / transplantation
  • Heterozygote
  • Homozygote
  • Humans
  • Mice
  • Mice, SCID
  • Mice, Transgenic
  • Molecular Sequence Data
  • Plasmids / genetics
  • Transplantation, Heterologous
  • Urokinase-Type Plasminogen Activator / genetics*


  • Urokinase-Type Plasminogen Activator