Abstract
SR-PSOX and CXC chemokine ligand (CXCL)16, which were originally identified as a scavenger receptor and a transmembrane-type chemokine, respectively, are indicated to be identical. In this study, we demonstrate that membrane-bound SR-PSOX/CXCL16 mediates adhesion and phagocytosis of both Gram-negative and Gram-positive bacteria. Importantly, our prepared anti-SR-PSOX mAb, which suppressed chemotactic activity of SR-PSOX, significantly inhibited bacterial phagocytosis by human APCs including dendritic cells. Various scavenger receptor ligands inhibited the bacterial phagocytosis of SR-PSOX. In addition, the recognition specificity for bacteria was determined by only the chemokine domain of SR-PSOX/CXCL16. Thus, SR-PSOX/CXCL16 may play an important role in facilitating uptake of various pathogens and chemotaxis of T and NKT cells by APCs through its chemokine domain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen-Presenting Cells / immunology*
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Antigen-Presenting Cells / metabolism
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Antigen-Presenting Cells / microbiology*
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Bacterial Adhesion / drug effects
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Bacterial Adhesion / immunology
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COS Cells
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Chemokine CXCL16
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Chemokine CXCL6
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Chemokines, CXC / chemistry
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Chemokines, CXC / metabolism
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Chemokines, CXC / physiology*
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Dextran Sulfate / pharmacology
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Escherichia coli / drug effects
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Escherichia coli / immunology
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Escherichia coli / metabolism
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Female
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Humans
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Ligands
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Lipoproteins, LDL / pharmacology
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Membrane Proteins / chemistry
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Membrane Proteins / metabolism
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Membrane Proteins / physiology*
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Mice
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Mice, Inbred BALB C
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Oxidation-Reduction
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Phagocytosis / immunology*
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Protein Structure, Tertiary / physiology
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Receptors, Immunologic / chemistry
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Receptors, Immunologic / metabolism
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Receptors, Immunologic / physiology*
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Receptors, Lipoprotein*
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Receptors, Scavenger
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Scavenger Receptors, Class B
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / immunology
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Staphylococcus aureus / metabolism
Substances
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CXCL16 protein, human
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Chemokine CXCL16
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Chemokine CXCL6
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Chemokines, CXC
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Cxcl16 protein, mouse
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Ligands
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Lipoproteins, LDL
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Membrane Proteins
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Receptors, Immunologic
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Receptors, Lipoprotein
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Receptors, Scavenger
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Scarb1 protein, mouse
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Scavenger Receptors, Class B
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oxidized low density lipoprotein
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Dextran Sulfate