Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides

Nucleic Acids Symp Ser. 2000;(44):87-8. doi: 10.1093/nass/44.1.87.

Abstract

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Damage*
  • DNA Glycosylases
  • DNA Repair
  • Deoxyribonucleotides / metabolism
  • Guanine / metabolism*
  • In Vitro Techniques
  • N-Glycosyl Hydrolases / metabolism
  • Nitric Oxide / toxicity*
  • Nitrogen Dioxide / toxicity*
  • Nucleotides / metabolism
  • Purine Nucleosides / metabolism
  • Pyrroles / metabolism
  • Xanthine / metabolism

Substances

  • 1-(2'-deoxyribofuranosyl)-3-nitropyrrole triphosphate
  • Deoxyribonucleotides
  • Nucleotides
  • Purine Nucleosides
  • Pyrroles
  • oxanine
  • Xanthine
  • Nitric Oxide
  • Guanine
  • DNA Glycosylases
  • N-Glycosyl Hydrolases
  • Nitrogen Dioxide