Abstract
Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA Damage*
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DNA Glycosylases
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DNA Repair
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Deoxyribonucleotides / metabolism
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Guanine / metabolism*
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In Vitro Techniques
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N-Glycosyl Hydrolases / metabolism
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Nitric Oxide / toxicity*
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Nitrogen Dioxide / toxicity*
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Nucleotides / metabolism
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Purine Nucleosides / metabolism
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Pyrroles / metabolism
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Xanthine / metabolism
Substances
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1-(2'-deoxyribofuranosyl)-3-nitropyrrole triphosphate
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Deoxyribonucleotides
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Nucleotides
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Purine Nucleosides
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Pyrroles
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oxanine
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Xanthine
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Nitric Oxide
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Guanine
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DNA Glycosylases
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N-Glycosyl Hydrolases
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Nitrogen Dioxide