Synthesis of non-natural DNA using DNA polymerase

Y Ebara; T Sugiyama; K Kaihatsu; S Ueji

doi:10.1093/nass/44.1.143

Synthesis of non-natural DNA using DNA polymerase

Nucleic Acids Symp Ser. 2000:(44):143-4. doi: 10.1093/nass/44.1.143.

Authors

Y Ebara¹, T Sugiyama, K Kaihatsu, S Ueji

Affiliation

¹ Division of Natural Environment and Bioorganic Chemistry, Faculty of Human Development and Sciences, Graduate School of Science and Technology, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe 657-8501, Japan.

PMID: 12903309
DOI: 10.1093/nass/44.1.143

Abstract

Nonnatural nucleotide modified by glucose or galactose was synthesized to increase functional diversity of DNA library. These compounds were incorporated in a DNA double strand using Klenow Fragment as well as dTTP. These functional group could be ordered sequentially on a DNA double strand at intervals of few angstroms according to the designed template sequence within a few hours. This method must be useful to constructing nonnatural DNA library or designed supramolecular structures.

MeSH terms

Base Sequence
DNA / biosynthesis*
DNA / chemistry*
DNA Polymerase I / metabolism*
Deoxyuracil Nucleotides / chemistry
Deoxyuracil Nucleotides / metabolism
Drug Design
Galactose / chemistry
Glucose / chemistry
Models, Molecular
Molecular Structure
Nucleic Acid Conformation
Substrate Specificity
Thymine Nucleotides / chemistry
Thymine Nucleotides / metabolism

Substances

Deoxyuracil Nucleotides
Thymine Nucleotides
deoxyuridine triphosphate
DNA
DNA Polymerase I
Glucose
thymidine 5'-triphosphate
Galactose