Effect of coxsackievirus b3 on ion channel currents in rat ventricular myocytes

Chin Med Sci J. 2000 Sep;15(3):150-3.

Abstract

Objective: To investigate the effects of coxsackievirus B3(CVB3) on ion channel currents in rat ventricular myocytes.

Methods: Rat hearts were isolated with collagenase to acquire single ventricular myocytes, L-type voltage-dependent calcium channel (VDCC) current (I(Ca)), Na+ current (I(Na)), outward potassium current (I(out)), inwardly rectifying potassium current(I(KI)) were recorded using whole cell patch clamp techniques.

Results: CVB3 infection increased I(Ca) and I(out), while decreased I(KI); but it had no obvious effect on I(Na).

Conclusion: The effects of CVB3 an I(Ca), I(out), I(KI) may be one of the mechanisms of myocytes damage and the occurrence of abnormal electroactivities induced by CVB3 infection.

MeSH terms

  • Animals
  • Animals, Newborn
  • Calcium Channels, L-Type
  • Cell Separation
  • Enterovirus B, Human* / pathogenicity
  • Enterovirus Infections / physiopathology*
  • Heart Ventricles / virology
  • Ion Channels*
  • Myocytes, Cardiac / physiology*
  • Myocytes, Cardiac / virology
  • Patch-Clamp Techniques
  • Potassium Channels, Inwardly Rectifying
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Channels
  • Ventricular Function

Substances

  • Calcium Channels, L-Type
  • Ion Channels
  • Potassium Channels, Inwardly Rectifying
  • Sodium Channels