[Effects of NBP on ATPase and anti-oxidant enzymes activities and lipid peroxidation in transient focal cerebral ischemic rats]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Feb;24(1):93-7.
[Article in Chinese]

Abstract

Objective: The aim of the present study was designed to explore the effect of (+/-) -3-n-butylphthalide (NBP) on ATPase, anti-oxidant enzymes activities and lipid peroxidation of mitochondria and cerebral cortex in rats subjected to 24 hours of reperfusion following 2 hours of cerebral ischemia (tMCAO).

Methods: Activities of SOD (Superoxide Dismutase), GSH-Px (glutathione Peroxidase,) and CAT (Catalase), and MDA level of mitochondria or cortex were measured by using biochemical methods in tMCAO rats.

Results: (1) The activities of mitochondrial Na+K(+)-ATPase, Ca(2+)-ATPase and Mg2+ ATPase were found to decrease significantly in the vehicle group (ischemia + saline). Pre-treatment with NBP (5, 10, 20 mg/kg, i.p.) 10 min before tMCAO markedly enhanced the activities of Na+K(+)-ATPase and Ca(2+)-ATPase, compared with vehicle group. (2) The activities of SOD and mitochondrial GSH-Px were decreased and MDA level increased in vehicle groups as compared with that in sham group (non-ischemia + saline). NBP (20 mg/kg, i.p.) significantly enhanced total mitochondrial SOD activity, and also enhanced cerebral cortex total SOD activity (in 5, 10, 20 mg/kg groups). However, it had no obvious effect on CuZn-SOD activity. NBP (20 mg/kg i.p.) markedly increased mitochondrial (but not in cerebral cortex) GSH-Px activity; NBP 10, 20 mg/kg markedly decreased mitochondrial MDA level compared with that in vehicle group (P < 0.05). (3) The action of raceme NBP on the increase of the activities of ATPase and antioxidative enzymes seemed to be beneficial than that of (-) -NBP or (+) NBP.

Conclusion: The results suggest that NBP improves energy pump and subsides oxidative injury which may contribute to its anti-neuronal apoptotic effect.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Benzofurans / pharmacology*
  • Cerebral Cortex / enzymology
  • Drugs, Chinese Herbal / pharmacology
  • Glutathione Peroxidase / metabolism
  • Ischemic Attack, Transient / enzymology*
  • Lipid Peroxidation
  • Male
  • Mitochondria / enzymology
  • Neuroprotective Agents / pharmacology*
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / enzymology
  • Superoxide Dismutase / metabolism

Substances

  • Benzofurans
  • Drugs, Chinese Herbal
  • Neuroprotective Agents
  • 3-n-butylphthalide
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Adenosine Triphosphatases