Broad-complex function during oogenesis in Drosophila melanogaster

Dev Genet. 1992;13(4):277-88. doi: 10.1002/dvg.1020130405.


The Broad-Complex (BR-C) appears to encode factors that mediate ecdysone effects during the larva-adult transition. The main goal of this study was to gain insight into what roles the BR-C might play during oogenesis. The main findings are as follows. First, as determined by heteroallele studies and clonal analysis, de12 is a somatic line mutation that appears to fall into the broad domain of the BR-C. Second, the de12 mutation is associated with the insertion of the gypsy transposon at position 169.5 (Chao and Guild, Embo J, 1986, 5:143-150) in the BR-C domain. In its new context this gypsy element exhibits ovarian-specific activation. Both this gypsy activation and the de12 phenotype are partially suppressible by su(f) and su(Hw). Third, we have identified a set of transcripts that cross-hybridize with BR-C sequence spanning the gypsy insertion site (166-179). There are significant differences in these cross-hybridizing species, both in size and relative abundance, between de12 and its parent strain. Finally we have determined that in de12 there is a premature arrest of chorion gene amplification in the late stages of oogenesis.

MeSH terms

  • Animals
  • Base Sequence
  • Chorion / physiology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / physiology
  • Ecdysone / physiology
  • Female
  • Genetic Complementation Test
  • Male
  • Molecular Sequence Data
  • Mutation / physiology
  • Oogenesis / genetics
  • Transcription, Genetic / physiology*


  • Ecdysone

Associated data

  • GENBANK/L02612
  • GENBANK/L02613
  • GENBANK/L02614
  • GENBANK/S44931
  • GENBANK/S44934
  • GENBANK/S55610
  • GENBANK/S55612
  • GENBANK/X62257
  • GENBANK/X62258
  • GENBANK/X62259