Alterations of the P16 gene in uterine cervical carcinoma from Indian patients

Int J Gynecol Cancer. Jul-Aug 2003;13(4):472-9. doi: 10.1046/j.1525-1438.2003.13330.x.

Abstract

In our analysis, alterations in the P16 tumor suppressor gene were seen in 33% (15/46) of sampled uterine cervical lesions. Among the alterations, mutations in P16 were detected in 15% (7/46) of the samples. One mutation occurred at intron 1/exon 2 splice junction. All the other mutations were in exon 2 with three of them as silent mutations. The promoter hypermethylation and homozygous deletion of P16 gene were detected in 6.5% (3/46) and 8.7% (4/46) of the samples respectively. Loss of heterozygosity and microsatellite size alterations at the P16 locus were seen in 17% (8/46) of the samples. HPV16/18 infection was detected in 76% (35/46) of the samples. But no association was found between P16 alterations and HPV infection. Thus, it seems that P16 inactivation may be associated with the development of some uterine cervical carcinoma.

MeSH terms

  • Adult
  • Aged
  • Alleles
  • Base Sequence
  • Biopsy, Needle
  • Carcinoma / genetics*
  • Carcinoma / pathology
  • Chi-Square Distribution
  • DNA, Neoplasm
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Genes, p16
  • Genetic Predisposition to Disease*
  • Humans
  • Immunohistochemistry
  • India
  • Middle Aged
  • Molecular Sequence Data
  • Mutation*
  • Neoplasm Staging
  • Polymerase Chain Reaction
  • Probability
  • Prognosis
  • Sampling Studies
  • Sensitivity and Specificity
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / pathology

Substances

  • DNA, Neoplasm