Transfer of pro-R hydrogen from NADH to dihydroxyacetonephosphate by sn-glycerol-1-phosphate dehydrogenase from the archaeon Methanothermobacter thermautotrophicus

Biosci Biotechnol Biochem. 2003 Jul;67(7):1605-8. doi: 10.1271/bbb.67.1605.


sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of the sn-glycerol-1-phosphate backbone of archaeal lipids. [4-3H]NADH that had 3H at the R side was produced from [4-3H]NAD and glucose with glucose dehydrogenase (a pro-S type enzyme). The 3H of this [4-3H]NADH was transferred to dihydroxyacetonephosphate during the sn-glycerol-1-phosphate dehydrogenase reaction. On the contrary, in a similar reaction using alcohol dehydrogenase (a pro-R type enzyme), 3H was not incorporated into glycerophosphate. These results confirmed a prediction of the tertiary structure of sn-glycerol-1-phosphate dehydrogenase by homology modeling.

MeSH terms

  • Dihydroxyacetone Phosphate / metabolism*
  • Glycerolphosphate Dehydrogenase / chemistry
  • Glycerolphosphate Dehydrogenase / metabolism*
  • Hydrogen / metabolism*
  • Methanobacteriaceae / enzymology*
  • Molecular Conformation
  • NAD / metabolism*


  • NAD
  • Dihydroxyacetone Phosphate
  • Hydrogen
  • Glycerolphosphate Dehydrogenase
  • glycerol-1-phosphate dehydrogenase